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Composition and bioactivity of the essential oils from Mosla chinensis against Aedes albopictus
CHEN Fei-Fei, PENG Ying-Hui, ZENG Dong-Qin, ZHANG Yun, QIN Qiao-Hui, HUANG Yi
Abstract1374)      PDF (556KB)(1175)      

Objective The bioactivity of the essential oils from Mosla chinensis against Aedes albopictus was determined, and the chemical composition of the essential oils was analyzed to provide the basis for development of botanical mosquito control agents. Methods The essential oils were extracted from M. chinensis using steam distillation. The bioactivity of the essential oils against 4th instar Ae. albopictus larvae and pupae was determined using immersion tests, and the repellent activity was examined using human skin tests. The gas chromatography?mass spectrometry was employed to analyze the chemical composition of the essential oils from M. chinensis. Results (1) The 24 h LC50 values for 4th instar Ae. albopictus larvae and pupae were 78.820 μg/ml and 122.656 μg/ml, respectively; (2) application of 1.5 mg/cm2 pure essential oils provided (2.330±0.167) h of complete protection time against adult mosquitoes, and 56% of the protective effects remained beyond 6 h; and (3) a total of 44 compounds were detected from the essential oils and 39 were identified, accounting for 98.67%. The main components included p?cymene (23.17%), 5-isopropyl-2-methylphenol (16.83%), thymol (14.92%), Benzene, 2-methoxy-4-methyl-1-(1-methylethyl) (12.89%), eucalyptol (8.48%), caryophyllene (3.96%), α?caryophyllene (3.16%) and thymol acetate (2.71%). Conclusion The essential oils from M. chinensis had strong bioactivities on both Ae. albopictus larvae and adult mosquitoes. New natural active compounds may be found from these essential oils for development of environment?friendly mosquito control agents.

2010, 21 (3): 211-214.
Bioassay of Pogostemon cablin (Blanoa) Benth oil against Aedes albopictus Skuse
ZENG Dong-Qin, PENG Ying-Hui, CHEN Fei-Fei, ZHANG Yun, QIN Qiao-Hui, HUANG Yi
Abstract1262)      PDF (464KB)(1306)      

【Abstract】 Objective To test the bioactivity of essential oil extracted from Pogostemon cablin (Blanoa) Benth against Aedes albopictus Skuse, so as to find a new kind of mosquito repellent. Methods Essential oil was extracted from P.cablin by steam distillation. The bioassay against larvae and pupae of Ae. albopictus was carried out by immersion method. The adulticidal of P.cablin against Ae.albopictus was investigated by modified flask method. The repellent activity of pure P.cablin oil to Ae. albopictus was  assayed  by  topical  bioassay method  on  the  skin.  Results (1) P.cablin  oil  had  strong  toxicity  to  larvae  and  pupae  of Ae.albopictus. The LC50  values  of  P.cablin  oil  to Ⅰ, Ⅱ, Ⅲ, Ⅳ instar  larvae  and  pupae of  Ae.albopictus were 20, 34, 59, 60 and 187 μg/ml after treatment for 24 h, respectively. (2) The fumigation efficacy of P.cablin oil to adult Ae.albopictus was quick and efficient, and the KT50 values against Ae.albopictus were 14.692, 16.528 and 14.807 min at the dosages of 18, 36 and 54 μg/cm3, respectively. (3) The 100% protection time offered by P.cablin oil on the skin were (2.70±0.12) h, (4.40±0.10) h and (6.00±0.44) h at dosages of 1.5, 2.0 and 2.5 mg/cm2, respectively. Conclusion P.cablin oil has good bioactivity to Ae.albopictus, which is a kind of potential environment?friendly mosquito?repellent.

2009, 20 (4): 335-338.